Hi Skyline team, I used to be a Skyline protein mode user but now switched to GC small molecule omics analysis. Current software I am using can only give me a laundry list of RT with precursor mass without any compound name information. I can import transition list and results file to Skyline but cannot annotate these peaks from RT. So I was trying to figure out how can I actually annotate this peaks using Skyline then output a list of compound peak area list.

So I was thinking to use NIST GC-MS library but clearly I have difficult time find the right one (my compounds are all volatile compounds with <350 m/z, but all library I can find is peptide size). So I was wondering can Skyline annotate those undefined Peaks from GC-MS (Agilent)? If yes, how can we actually do it using Skyline? Thank you very much!